16-substituted 1, 3-diacyloxyestra-1, 3, 5(10)-trien-17-ones



United States Patent 3,024,256 IG-SUBSTITUTED 1,3-DIACYLOXYESTRA-1,3,5(10)- TRlEN-17-ONES .lames Jiu, Morton Grove, IlL, assignor to G. D. Searle & Co., Chicago, Ill., a corporation of Delaware No Drawing. Filed Feb. 20, 1961, Ser. No. 90,231 5 Claims. (Cl. 260--397.4)

This invention relates to 1,3-diacyloxyestra-l,3,5(10)- trien-l7-ones and 1,3-dihydroxyestra-l,3,5(10)-trien-l7- ones which are substituted in the 16-position by a hydroxy or by a halogen. More particularly, this invention relates to products of the formula wherein R is hydrogen, lower alkanoyl, or benzoyl; X represents a member of the group consisting of hydroXy and halogen of atomic number greater than 16. Thus, the substituent in the 16-position can be hydroxy, chlorine, bromine or iodine.

The compounds of this invention are useful because of their valuable pharmacological properties. They have, for example, the capacity to decrease the serum con centration of cholesterol and the corresponding cholesterol/phospholipid ratio. They are also anti-anabolic agents and weak estrogens.

The 16-substituted 1,3-diacyloxyestra-1,3,5(10)-trienl7-ones hereof are prepared by reacting a 1,3,17-triacetoxyestra-1,3,5(l),16-tetraene with an appropriate reagent which will react with the double bond in the 16-position. Thus, reaction of the above tetraene with a halogen gives the corresponding 16a-halo-l,3-diacyloxyestra-l,3,5(10)- trien-17-one while reaction of the tetraene with perbenzoic acid gives 16oc-hydroxy-1,3-diacyloxyestra-1,3,5(10)- trien-17-one. When these compounds are allowed to stand with methanol in the presence of hydrochloric acid, the corresponding 1,3-dihydroxy compounds are formed.

The following examples describe in detail certain of the compounds illustrative of the present invention and methods which have been devised for their manufacture. However, the invention is not to be construed as limited thereby, either in spirit or in scope, since it will be apparent to those skilled in the art of organic synthesis that many modifications, both of materials and of methods, may be practiced without departing from the purpose and intent of this disclosure. In the examples hereinafter detailed, temperatures are given in degrees centigrade C.) and relative amounts of materials in parts by weight, except as otherwise noted.

Example 1 1 60L'Chl0rO'J ,3-diacet0xyestm-1 ,3,5 (10) -trien-1 7-0ne.-- A mixture of 1.5 parts of 1,3,l7-triacetoXyestra-1,3,5(l0), l6-tetraene (preparable by the procedure of Example 1 in U.S. 2,949,477) and 1.2 parts of potassium carbonate in 192 parts of carbon tetrachloride is cooled to 0 C.

3,024,256 Patented Mar. 6, 1962 action mixture is stirred for an additional minutes, it

is poured into water containing some sodium sulfite and extracted with chloroform. The combined chloroform extracts are washed with water, dried over anhydrous sodium sulfate and evaporated to dryness under reduced pressure. The residue is chromatographed on fullers earth using ethyl acetate and benzene as developing solvents. Elution with 1 to 5% ethyl acetate in benzene (v./v.) and recrystallization of the resultant product from methanol gives 16a-chloro-1,3-diacetoxyestra-1,3,5(10)- trien-17-one melting at about 178-180 C. The product has the formula --o1 on ooo OHQCOO Example 2 I6a-br0mo-1,3-diacet0xyestra-1,3,5 (-10)-trien-1 7-0n'e. To a solution of 1.5 parts of 1,3,l7-triacetoXyestra-l,3, 5 (l0),l6-tetraene in 192 parts of carbon tetrachloride is added 1.2 parts of potassium carbonate. The mixture is stirred and cooled in an ice-water bath. A solution of bromine in carbon tetrachloride is added to the reaction mixture in small portions over a period of about 10 minutes until the bromine coloration persists for about a minute. The carbon tetrachloride solution is poured into water containing some sodium sulfite and the resultant mixture is extracted with chloroform. The combined chloroform extracts are washed with water, dried over anhydrous sodium sulfate and evaporated to dryness under reduced pressure. The residue is chromatographed on activated magnesium silicate using ethyl acetate and benzene as developing solvents. The product obtained by eluting with 5 to 20% ethyl acetate in benzene (v./v.) is 16a-bromo-1,3-diacetoxyestra-l,3,5(10)- trien-17-one which, after recrystallization from methanol, melts at about 157-l60 C. The product has the formula --Br CHsCOO Substitution of 1,3,17-tribenzoyloxyestra-1,3,5(10),16- tetraene for the 1,3,17-triacetoxyestra-1,3,5(10),16-tetraene in the above procedure gives 16a-bromo-1,3-dibenzoyloxyestra-1,3,5(10)-trien-17-one.

Example 3 benzene. The reaction mixture is allowed to stand at room temperature for 18 hours before diluting it with benzene. The benzene solution is washed with cold dilute sodium hydroxide solution and cold sodium chloride solution. After drying the solution over anhydrous sodium sulfate, the solvent is removed under reduced pressure. The residue is recrystallized from methanol to yield 16ot-hydroxy-1,3-diacetoxyestra-l ,3,5( lO)-trien-l7- one melting at about 2l02l5 C. This product has the following formula CHaOOO Example 4 4 What is claimed is: 1. A compound of the formula wherein R is selected from the group consisting of hydrogen, lower alkanoyl, and benzoyl and X is selected 15 from the group consisting of hydroxy and halogen of atomic number greater than 16.

2. A compound of the formula --Y CHaCO? CHQCOO wherein Y is a halogen of atomic number greater than 16. 3. 1Goa-chloro-1,3-diacetoxyestra-1,3,5(10)-trien-17-one. 4. 16a-brom0-1,3-diacetoxyestra-1,3,5(l0) trien 17- one.

5. 16a-hydroxy-1,3-diacetoxy-1,3,5(10)-trien-17-one.

No references cited. 

1. A COMPOUND OF THE FORMULA 